Place it at the reveal, after the setup has played out and just before the consequences are shown. Follow it with a sad trombone for a slow, comic defeat, or a record scratch if the moment needs a hard stop, then let the next clip carry the reaction forward.
关于此声音
This is a spoken reaction clip lasting about ten seconds, centred on one alarmed, stretched-out 'oh no' in a voice that sounds suddenly uneasy. It gives a listener an awkward, doomed feeling, making a small mistake land as much more embarrassing.