The clip comes from user generated soundboard culture, where very short anime style vocal reactions are saved under the name of the person saying them and a number for a repeat upload. Its exact recording source is not documented, so its life has been online rather than in a film or series. Short uwu sounds spread because they work as text free reactions in chats, edits and streams, standing in for shyness or affection in less than a second. This version stays in use for that quick, recognisable punchline effect.