Place it at the end of a build-up, just before the answer to the request, then follow a refusal with the Law and Order Dun Dun sting to close the joke. For a softer ending, let a sad song take over instead of another voice clip.
关于此声音
This clip is Rachel Berry from Glee delivering a short, pleading line in her earnest, theatrical voice, lasting just under ten seconds. It feels funny and overdramatic, the kind of desperate begging that turns a small request into a big moment. It comes from Glee.