Place it at the end of a repeated request, after the ask has been shown twice, then follow it with the Bruh sound for a reaction. If the requester keeps pushing, add the Oh My God Bro Oh Hell Nah sound before it to build the refusal, or use the Emotional Damage sound after it as the final reply.
关于此声音
This is a short spoken clip, lasting about five seconds, of Italian musician Richard Benson saying 'questa volta no', or 'this time no' in English. It gives a blunt, comic feeling of refusal, with the short phrase making the no sound final and a little theatrical. The tone is defiant rather than angry, so it suits a repeated request that deserves a firm answer.