The line comes from The Backyardigans, the Nickelodeon animated series that premiered in 2004 and followed five friends staging musical adventures in their shared backyard. Tyrone, the orange moose in the group, delivers the museum line in character during one of those pretend scenarios. Clipped on its own, the moment circulated on soundboards and in short edits because the rule sounds oddly specific and the delivery is instantly quotable. It remains popular for reaction videos where a strict, funny no is funnier than a plain explanation.