Put it at the end of a build-up, after Sudden Suspense Sound Effect has raised the tension, so the reaction becomes the payoff. Follow it with Oh My God Wow for a second reaction, or Nelson Ha Ha Sound Effect when the joke needs a mocking finish.
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This two-second clip is Tamil comic actor Vadivelu shouting 'adampudikiriye' in a sharp, high-energy burst of disbelief. It feels funny, panicked and deliberately overblown, turning a small surprise into a comic gasp. Taken from his Tamil film comedy, it is instantly recognisable to fans even without any picture to go with it.