Place it at the end of a failed comeback sequence, after the danger has passed and the letdown is clear. Build tension first with Sudden Suspense, let this clip deliver the verdict, then follow it with Damn Meme for a sharper reaction or Nelson's Ha-Ha! if the mockery should continue.
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This short spoken clip delivers the line about what does not kill you disappointing the speaker, stretched across just over three seconds. It lands as a dry, sarcastic letdown, funny because the expected toughness turns into plain disappointment instead. The unimpressed delivery makes it feel like a sigh at someone who survived trouble and still managed to underwhelm.