Place it just after the twist is revealed and before the reaction shot, so the villain's moment stays the focus. Open with It Was At This Moment He Knew to set up the turn, then follow this clip with Darth Vader Nooo for a bigger villain payoff.
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This short clip is Woody's villainous laugh pulled down to a slower, deeper pace, turning the familiar cowboy's chuckle into a drawn-out cackle that lasts just over two and a half seconds. It feels gleefully mischievous and deliberately over the top, like a cartoon bad guy savouring a plan. The laugh comes from Toy Story, where Woody delivers it in character.