Place it at the exact frame the joke turns on you, then follow the protest with the awkward moment sound effect for the fallout, or precede it with the anime ahh sound effect to build the panic before you speak.
关于此声音
This clip is a spoken anime-style voice line saying "yamete kudasai," the Japanese phrase for please stop. It feels flustered and dramatically embarrassed, turning a small protest into an over-the-top reaction. The phrase is common across anime and manga and in anime-inspired meme culture, rather than tied to one confirmed source work.