Put it at the end of a reaction beat, just before the next scene starts, then follow it with the Tuturu sound for a second anime reply, or use the Anime Ahh sound before it to build up the surprise.
关于此声音
This is a 0.7-second voice clip of Yuudachi asking “poi?” in a bright, rising anime voice. It feels cute, playful and a little confused, like a sweet reaction to something unexpected. The line comes from Kantai Collection, where Yuudachi uses “poi” as her signature speech quirk.