Place it immediately after the visual cause in your edit, like a dice landing or a token moving, then follow it with a laugh track or a sad trombone if you want the setback to play as a running gag across the video.
關於此聲音
This is a very short vocal clip of someone saying 'ow', lasting just 0.65 seconds as a single sharp reaction. It gives a quick, pained but playful feeling, like a small sting or setback that is more funny than serious, making the hurt sound over the top for such a brief moment.