Place it at the exact frame a vehicle blocks the lane, before any reaction line from your characters. Follow it with MLG Air Horn for a bigger pile-up gag, or lead into it with Vine Boom when a reveal needs a beat right before the honk moment hits.
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A single loud truck horn blast that lasts about a second and a half, with the deep, brash tone of a big rig on the road. It feels abrupt and attention-grabbing, a little rude and funny at the same time, like being honked at out of nowhere in traffic.