Place it as the payoff beat after a setup sound, not as the opener. Lead with the Mario Jump Sound Button for the lift, follow with this clip on the landing, and save the SpongeBob Fail Sound Button for a second, sadder follow-up joke.
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This clip is a short 1.2-second cartoon boing, a springy stretched twang made for classic slapstick gags. It gives a listener a light, silly and instantly playful feeling, turning a small stumble or bounce into a harmless joke rather than a painful accident.