Put it right on the reveal, after a Vine Boom Sound to set up the entrance and before an Anime Wow to catch the reaction, so the horse lands as the middle beat of a three-part gag.
關於此聲音
Cheval is a brief horse sound effect lasting about one and a half seconds, built around a single neigh. It feels daft and unexpected, the kind of noise that makes people laugh because a horse has no business appearing in the conversation at all.