The line comes from The Room, the 2003 independent drama written, directed by and starring Tommy Wiseau as Johnny. In a rooftop confrontation Johnny accuses his friend Mark of being a chicken and launches into the cheeping impression. The film became a cult phenomenon through midnight screenings and later The Disaster Artist, and this moment became a shorthand meme for cowardice. Clips circulate widely on soundboards and in edits because the performance is instantly recognizable even without any visual context from the original scene today.
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Tommy Wiseau as Johnny doing his chicken impression from The Room, repeating cheep cheep cheep. Used in memes and edits to mock someone as cowardly or scared.
This is Tommy Wiseau as Johnny in The Room delivering his bizarre chicken impression, repeating cheep cheep cheep in a flat, childlike voice while flapping his arms and bobbing his head. The delivery is completely dead serious, which makes the childish noises sound stranger and funnier with every repetition. There is no music or build up, just Wiseau committing fully to the bit for several seconds. Viewers instantly feel the awkward tension of the scene, and the sheer absurdity turns a confrontation about betrayal into one of the most quoted and parodied moments in cult cinema.