Place it immediately after the mistake is shown, so it becomes the reaction to that moment rather than a separate joke. Follow it with another surprised reaction sound for contrast, then return to the main action without repeating the same beat.
關於此聲音
This short clip presents the D'oh exclamation in Japanese, lasting about four seconds as a single spoken reaction. It is brief and direct, with a light, amused feeling that fits a small mistake or an awkward slip. It works as a quick vocal response rather than a longer line, keeping the focus on the familiar sound itself.