Place it just before the two sides meet on screen, then follow the stare with JoJo To Be Continued for a cliffhanger ending, or cut to Awkward Moment Sound Effect when neither side backs down.
關於此聲音
This is a short, just over a second long whistle taken from the main theme of The Good, the Bad and the Ugly, a single clear tune with no words or instruments behind it. It feels tense and playful at once, turning an ordinary pause into a mock showdown. The melody was written by Ennio Morricone for Sergio Leone's 1966 western.