Place it just before the reveal, then follow it with Sudden Suspense and Oh My God Wow for the payoff. If the scene turns into a panic, put No No Wait Wait right after it so the hesitation becomes the joke.
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Instant Tico Nervoso is a very short reaction clip lasting about 1.4 seconds, built around a jittery, nervous tic that lands in a single quick burst. It feels tense, fidgety and awkward in a funny way, like catching someone mid-flinch before they can compose themselves. That uneasy energy makes it useful for moments of panic, embarrassment or sudden nerves.