Put it at the end of a bathroom fail sequence, after a classic fart sound and before a SpongeBob fail sting, so the accident becomes the climax and the fail reaction closes the bit. A second fart meme sound works earlier in the build-up, but keep this clip as the payoff.
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A man on a toilet lets out a loud, strained scream as he sharts, the whole messy moment lasting just over five seconds. The raw vocal burst and the unmistakable bathroom accident make it crude, shocking and hard not to laugh at, especially when it catches someone off guard. It works as pure gross-out comedy, awkward in the best possible way for a prank.