Place it at the end of a fail sequence, after the mistake is clear, then follow it with Emotional Damage Meme Sound to underline the defeat, or open with Game Show Buzzer Sound Effect before the fall to set up the miss.
關於此聲音
A 25-second game clip lets Mario's panic stretch out, with a rising, wavering cry that keeps going until he is gone from view. The effect is silly and overblown, so a defeat feels bigger and funnier than it should. Mario is the plumber hero Nintendo has used for decades, and editors keep returning to his voice when they need a defeat to feel dramatic.