Place it at the end of a sad setup, after a Sad Meow Song cue, so the turn lands before the joke changes direction, then follow it with Emotional Damage Meme Sound to underline the defeat without repeating the same gag.
關於此聲音
This 3.6-second clip takes a slow, mournful melody and pushes it through heavy distortion until it sounds loud, harsh and deliberately overwhelming, with the familiar sad tune still recognisable underneath the clipping. The effect is funny and jarring at once, turning a tender moment into an abrupt comic shock that makes a fail or rejection feel sadder and funnier together.