Put it just after the setup moment and before the payoff, letting it bridge a failed plan and the next joke. Follow it with Nope to shut the idea down, or open with Okay Let's Go before the plan goes wrong.
關於此聲音
This is a sub-second voice clip of Sojiro Sakura from Persona 5 saying 'Hoo boy' in a dry, worn-out tone. It feels amused and exasperated at once, like a sigh for a situation that is awkward, tiring or mildly ridiculous. The line comes from Atlus's role-playing game, released in 2016, where Sojiro's calm, world-weary manner makes even a two-word reaction stick.