Place it immediately after the harsh line or betrayal in your edit, then follow it with the Awkward Moment Sound Effect to let the fallout sit. For a different follow-up, use the SpongeBob Disappointed Sound instead, keeping this clip as the accusation in the middle of the sequence.
關於此聲音
This is a short Japanese voice clip, about two seconds long, of a speaker asking 'hito no kokoro toka nain ka?' — 'do you have no heart?' The delivery sounds shocked, hurt and accusing at once, so the line feels both funny and painfully unfair. It lands as a sharp reaction to a cold remark, a betrayal or a joke that goes too far.