Place it exactly on the frog reveal frame in your edit, not during the intro or outro. Build toward it with the dun dun dun sound effect for a fake serious lead-in, then follow it immediately with a vine boom sound button to close the gag before cutting to the next scene.
關於此聲音
This clip is just over a second long and features a plush froggy toy letting out a sudden, high screamer yell. The effect is startling at first, then funny, because the cute plush look clashes with the frantic noise coming from it and turns a harmless frog into a tiny jump scare.