Place it at a hard cut between two clips, just before the next scene begins, to mark the change of speaker. Follow it with a short laugh for a light payoff, or put a game-show correct-answer sting after it when the shut-down proves a point.
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This is a very short whip crack, lasting under a second, with a single sharp snap that cuts through the air. It feels abrupt, cheeky and dismissive, like a quick verbal slap for someone who will not stop talking. The sudden sting makes it funny rather than harsh, landing as a playful shut-down instead of a real scolding.