Place it at the end of a fail sequence, right after the mishap, then follow it with SpongeBob Sad Song for the aftermath, or swap in Lego Yoda Death Sound when the defeat needs a second punchline in a game edit.
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This under-two-second clip is Squidward Tentacles letting out a high, cracking scream as something goes badly wrong for him in SpongeBob SquarePants. It feels like sudden, over-the-top panic, funny rather than frightening because the grumpy neighbour is always the one suffering. The clip circulates as his defeat sound in MUGEN fighting games and is linked to the episode Squidtastic Voyage.