Put it at the end of a short frog gag, after the setup lands and just before the reaction shot. Pair a classic fart first for a bigger build, then let this clip deliver the smaller payoff, and finish with a vine boom if the joke needs a louder close.
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This is a very short, just over one second long sound effect of a squished frog letting out a small wet fart, with a soft squelchy squeeze under the toot. It lands as silly and gross in a harmless way, the kind of noise that makes people laugh before they can stop themselves. It works best as a quick surprise, not a drawn-out joke.