Place it at the very end of a failed attempt, after the mistake is shown, so it closes the scene. For a bigger defeat, follow it with the Mortal Kombat Fatality cue, or precede a slow recovery with the SpongeBob Sad Song before the next attempt begins.
關於此聲音
This is the short game-over jingle from Super Mario Bros, lasting just under eight seconds with a simple descending melody that lands on a flat, finished note. It feels instantly deflating and comic, turning a lost attempt into a shared joke. The tune comes from Nintendo's original game, where it played after losing your last life.