Place it near the end of a night scene, after Dun Dun Dun sets up the reveal and before Vine Boom lands the punch, so the sequence moves from setup to the moment, then cuts straight to the reaction.
關於此聲音
This is a wolf sound effect lasting just under eight seconds, centred on a wolf call heard in the dark. It feels eerie and lonely, like something is watching from the trees, and it works best when the listener has a moment to picture the animal before anything else happens.