Place it at the turning point of a gaming edit, after your establishing shots and before the reaction beats. Follow it with a character death sound for a mishap, then return to your normal gameplay footage to close the gag.
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This 7-second clip is the lobby music from Welcome to Bloxburg, remixed into a heavily boosted, distorted earrape version. The recognisable calm melody hits as a harsh blast instead, giving listeners a startled, prank-like jolt that feels funny because the original was so peaceful and familiar while waiting to load in.