Place it right after the setup line in your edit, then follow it with the Bruh Sound Effect before cutting to the next scene, or pair it with Emotional Damage Meme Sound when the remark was aimed at someone.
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This two-second clip is a quick, confrontational reaction line spoken with a sharp, incredulous voice and a sudden pause that makes the challenge land hard. It feels instantly offended and disbelieving, turning a bold or out-of-pocket remark into a joke in one beat. It comes from The Boondocks, the animated television series that first aired in 2005.