Place it at the landing point of a jump or drop in your edit, preceded by the Mario Jump Sound Button as the character leaves the ground and followed by the SpongeBob Fail Sound Button when the landing goes wrong on purpose.
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This is a very short sound effect built from two heavy, chunky thuds in quick succession, lasting just over a second. The double hit gives it a silly, weighty feeling, like something oversized has stomped or landed, and that exaggerated heaviness is what makes the moment feel funny rather than serious.