Place it at the reveal in a prank or horror edit, after the setup has built tension, then cut straight to the reaction. Follow it with Oh My God Meme for the reaction, or stack Scream Meme straight after it for a double scare in a fast-paced clip.
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This clip is a short, sharp human scream, high-pitched and sudden, like someone reacting to a fright in the dark. It lands as startling and tense for a split second, then funny once the shock passes, which makes it work as a jump scare rather than a serious cry for help.