Place it at the exact frame of the fail or insult reveal in your edit, not before it. Follow it with the original Emotional Damage version for a double hit, or precede it with a Bruh Soundboard Button cue when the mistake needs a quiet setup first.
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This bass boosted clip is a short 3-second shout of a man yelling Emotional Damage with the low end pushed up for extra punch. It turns a small embarrassment or harsh comment into a big, theatrical reaction, making the hurt feel funny and overblown rather than actually sad or serious.