Place it at the end of a build-up, once the twist is clear, and let it mark the turn. Open with the Inception Horn for the build-up, then use this laugh as the reveal, and follow it with the FNAF Jumpscare Scream when the scare actually lands.
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This two-second clip is a sharp, high laugh with a taunting edge, the kind of sound that cuts through a scene in an instant. It feels mischievous and menacing at once, turning a joke into something ominous and a reveal into a threat. It comes from The Legend of Zelda: Majora's Mask, the Nintendo 64 game released in 2000, where the masked antagonist gives the game its unsettling mood.