Place this clip right at the reveal in your edit, after a fail sound meme button sets up the mistake. Follow it with a bruh meme button for the group's reaction, then cut straight to the next scene without layering anything underneath.
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This clip is Darth Vader stretching a single anguished 'Nooooooo' into a long, deep-voiced scream that lasts about six seconds. It lands as pure melodramatic despair, the kind of over-the-top heartbreak that turns a small disappointment into a huge tragedy, which is exactly why listeners find the exaggeration so funny.