The sound comes from Star Wars Episode III Revenge of the Sith from 2005, in the scene where the newly armored Darth Vader learns Padme Amidala has died and screams in grief. The moment became one of the most parodied scenes in the prequel trilogy, spreading through YouTube edits, forums and meme videos in the late 2000s. Also shared as Darth Vader NOOOOOOOOO clips, it endures because the over the top delivery turns any disappointment into a joke about total despair.
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Darth Vader's dramatic Nooooooo scream from Star Wars Revenge of the Sith, used in memes and edits for epic fails, bad news and over the top reactions.
This is Darth Vader's long, anguished Nooooooo after he learns Padme has died in Star Wars Episode III Revenge of the Sith. Delivered through his mask with heavy breathing underneath, the cry starts low and desperate then rises into a raw, echoing wail that lasts several seconds. It is not scary so much as painfully dramatic, which is why it lands as both tragic and funny out of context. Played over a small mistake, it instantly makes the moment feel like a huge, galaxy sized loss.