Place this clip right after the mistake becomes visible, then follow it with a fail sound meme for a second, smaller punch, or lead in with It Was At This Moment He Knew before switching to Vader for the payoff.
About this sound
This short clip is Darth Vader shouting a stretched-out "Nooo" in his deep, mechanical voice, lasting just over a second. It feels melodramatically devastated and instantly funny, turning a small loss into pure tragedy. It comes from Star Wars: Episode III – Revenge of the Sith, from the moment Vader learns Padmé has died.