Place it at the exact cut where a new person appears, after a Discord Join cue and before an Oh My God Wow reaction if the moment escalates. For smaller beats, keep it alone as the only reaction in that scene.
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This is a very brief spoken clip of a voice saying 'Oh, hi' in under a second. The delivery lands as a surprised but friendly greeting, like noticing someone mid-thought, which gives it a light, slightly awkward and instantly recognisable reaction feeling.