Place it at the start of a reaction sequence, right after the unbelievable claim, so it frames everything that follows. Follow it with a Bruh sound for a dismissal, or an Emotional Damage sound when the claim deserves a bigger reaction. Keep it as the first challenge before any punchline sound.
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This is a short spoken line, lasting about a second and a half, in which a male voice asks 'What chu mean, my boy?' in relaxed, slang-heavy delivery. The question lands as playful disbelief, the kind of half-laughing challenge you give when a claim sounds wrong or a story stops making sense. It feels cheeky and confrontational without turning hostile, so the confusion itself becomes the joke.