Place it the moment the intruder appears on screen, then follow it with the Metal Gear Alert Sound to raise the stakes before cutting to the reaction. For a courtroom-style payoff, end the sequence with the Law & Order Dun Dun instead.
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This 1.3-second clip is Old Gregg, a flamboyant merman-like creature, asking in a deep, slurred voice what someone is doing in his waters. The delivery feels possessive and faintly threatening, yet absurd enough to make the challenge funny rather than frightening. It comes from The Legend of Old Gregg, a 2005 episode of The Mighty Boosh, where he confronts Howard Moon after being hooked.