Place it directly after the failed play in a gaming montage, then follow it with the Mission Failed sound to underline the defeat, or with Ba Dum Tss when the miss was more embarrassing than serious.
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A two-second Waluigi voice line from Mario Party 7, spoken in his sneering, over-the-top tone as he says 'You're lousy.' It feels like a lighthearted taunt, funny and smug enough to sting for a second without turning mean. The line comes from the GameCube party game, where Waluigi uses it to mock a rival after a failure.