Place it immediately after the line where someone makes the proposal, as the edit's turning point. Follow it with awkward crickets when the rejection needs an empty beat afterwards, or put a record-scratch style stop before it in fast meme edits.
Über diesen Sound
This is a very short voice clip, under a second long, built around a single blunt 'no' delivered as a flat rejection. It feels funny and dismissive, like shutting an idea down before it can even get started properly. The clip is tied to Alejo y Valentina, the animated series named in the upload description.