Place it at the very start of a scene as the entrance cue, then follow the reaction with a bruh hit if the attempt fails instantly. For a bigger build, let it lead into a meme sting rather than layering other music underneath it.
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This clip is just the first line of Megalovania, the driving opening notes played in about one second with its sharp synth lead. It feels instantly tense and mischievous, like a boss fight has started before anyone is ready. The track comes from Undertale, where it plays during the fight against Sans.