Plankton is the main villain in SpongeBob SquarePants created by Stephen Hillenburg for Nickelodeon and this scream comes from the season five episode Mermaid Man vs SpongeBob. In the episode Plankton tries to control Mermaid Man to attack the Krusty Krab. The clip later spread on soundboards and in meme edits alongside other Bikini Bottom staples like the spongebob sad song and the My leg! gag from Fred the fish. Its short length and instantly recognisable voice keep it in rotation for reaction videos.