GLaDOS was introduced in Valve's Portal in 2007, voiced by Ellen McLain as the polite but murderous AI running the Aperture Science test chambers. Clips of her voice spread through gaming videos and forums because her flat delivery makes even panic sound calculated. This repeated no became a reusable reaction separate from the game, shared in edits and streams whenever a player or character refuses an outcome. It remains popular because Portal is widely quoted and the line needs no context to understand.