Place it right after the unanswered message appears on screen, then follow it with the sad violin, or cut to Nelson's laugh when the reply that never came is the joke itself.
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This is a short spoken clip of a voice calling out 'Ignorado!' — Portuguese for 'Ignored!' — in a flat, dismissive tone that lands in just under three seconds. It feels blunt and funny, like a verbal shrug after a message goes unanswered, turning a small snub into a punchline. The clip is credited to Tio Orochi.