Place it right after the confusing line in an edit, before the reaction shot, so the misunderstanding drives the cut. Follow it with the Nani Sore sound for a second reaction, then let the next line land on its own.
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This is a very short spoken reaction built around a single questioning word, delivered in a quick, rising tone that sounds caught off guard. It lands as pure confusion, the kind of baffled pause you give when a statement does not quite add up. Listeners hear it as funny and awkward at once, a tiny interruption that makes a strange moment feel even stranger.