The sound comes from Dota 2, Valve's multiplayer online battle arena game, where Wisp is the in-game name for the hero Io. In Dota 2, a deny happens when a player kills their own creep to stop the enemy gaining gold and experience, and heroes have short voice lines tied to that action. Clips like this spread through Dota communities, soundboards and short-form videos because the mechanic is instantly recognisable to players. It remains popular as a quick, knowing joke for lane highlights, memes and streams.