Put it in the middle of a short edit, just after the setup line and before the reaction shot, then follow it with the Vine Boom sound for a heavy punch and the Anime Wow sound when a character reacts in surprise.
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This is a very short helium voice effect, lasting just over half a second, with a thin, high-pitched tone like a voice after breathing helium. It feels silly and surprising, turning a straight line into something playful in an instant. The effect was made on 11 February 2015.